Nov 30, 2012

Sterile filtering

        17. Warm the solution in hot water for 5-10 min. Draw 10ml oil out of the vial/container holding your gold or yellow colored solution. See V3 for an alternative to drawing.

      18. Turn the syringe upside down.

     19. Remove the needle.

     20. Open the sterile filter package, but leave the filter in the plastic!

     21. Holding the filter from the side through the plastic, screw the sterile syringe filter onto the syringe.

     22. Remove the stopper from vial 3 and place it into the now empty sterile syringe container. (See V4 for an alternative for which you don't need to remove the stopper.)

    23. Place the syringe filter/syringe combination immediately onto the mouth of the sterile vial ‘3'.